Investigating Effect Essay Example for Free

Investigating Effect Essay Plan Aim: The aim of the experiment is to find out what effect temperature has on the action of a protease enzyme on exposed developed film. Enzymes are biological catalysts. They are made in livings things built up by amino acids to make protein. Enzymes are able to speed up reactions and can repeat reactions. There are various factors that affect the activity of enzymes they are: Y Temperature Y pH Y Specificity Y Concentration of enzyme or substrate Enzymes are specific, this means that they only work on one substrate molecule. A substrate molecule is what the enzyme actually works on. The factors I have chosen to investigate are temperature. This therefore means that the temperature will be the independent variable. In the experiment there will be a transparent plastic backing of developed film, which will have a black gelatine coat on it. The gelatine coat is protein, which is the substrate molecule. I will put the film into protease solution, which is the enzyme. By having the gelatine coat I am able to see what happens to the gelatine coat when the temperature increases. I can find out if temperature affects the action of a protease enzyme. Prediction: Enzymes have an optimum temperature, which is generally below 400C. The optimum temperature is when enzymes works best and fastest at. When the temperature rises the rate increases. This is because the substrate and enzyme molecules are moving faster because the temperature has increased. This means that the molecules have more energy. They therefore are likely to collide more often with each other and a reaction will take place. However if the temperature goes over the optimum temperature the reaction slows down and the enzyme denatures. This means that it has changed shape and therefore the substrate can no longer fit into the enzyme. The diagram below shows how the substrate molecules which is protein fits into the enzyme, which is a protease molecule. This type of mechanism is called the lock and key hypothesis. If the active site, which is the enzyme, is heated too much it will change shape and no longer fit the substrate. The substrate therefore no longer is able to react if there is no active enzyme. I predict that when the temperature increases the time taken for the gelatine to be broken down will decrease. This is because temperature is a catalyst, which helps to speed up the enzymes, which are biological catalysts. When the temperature is 300C I predict that it will take longer for the film to become transparent than when the film is in a temperature of 600C. However at a certain temperature in the experiment I predict that there will be an optimum temperature. This is when the enzyme works best at. After this point the enzymes start to slow down and eventually denature which means it is harder for the substrate molecules to fit into the enzyme molecules. As I predict that when the temperature increases the time taken for the gelatine to be broken down decreases until it reaches the optimum temperature I therefore predict that the rate of reaction will increase when the temperature increases until it reaches the point when the enzymes start to denature. When the temperature is increased the enzyme molecules will break down the black gelatine coat quicker and therefore the developed film will become transparent faster. When temperature is increased the substrate molecules of protein will collide more frequently with the enzyme molecules. So if the temperature is increased from 300C to 600C the enzyme molecule will break the black gelatine down faster to leave the transparent plastic backing. The two diagrams show the effect of temperature between substrate molecules and enzyme molecules. They are only rough diagrams of what will happen between the two molecules. Y Substrate molecule- Y Enzyme molecule- Method: Apparatus: The apparatus that I am going to use for the experiment will be a test tube, developed film with a gelatine coat, splint, syringe, stopwatch, thermometer and electric water baths. This equipment is suitable for this experiment because it is easily available, it is easy to set up and use and it is easy to collect results with. This is how the experiment will be set up I will firstly measure the volume of protease solution by using a syringe, which will be 10cm3 and then put it into a test tube. I will then get two developed films and hook wire onto each so I am able to get them out of the tube easily. The wire will be labelled so it is easy to see which film is which. I will then put the test tube into an electric water bath, which is at a specific temperature for example 300C. I will leave it in the bath for three minutes and then put the two films into the test tube. Every 30 seconds I will check to see if the film has become transparent. When the two films have become transparent I take them out of the test tube. I then check  the pH of the protease solution by getting a glass rod and dipping it into the solution and then put the solution onto pH paper. Preliminary experiment: For my preliminary experiment I set up the apparatus as above. As it was only preliminary I used one film. I chose two temperatures to put two test tubes of protease into, they were 600C and 300C. I put the two test tubes into the two different electric water baths and then after three minutes put film in each. This is how the results turned out: Temperature of water bath/0CTest tube in water bath with no developed film/secsTime taken for film to become transparent/secsRate of reaction/ 1/secs (S-1) 301808000.0013 601803000.0033 This table of results indicates that when the temperature increases the time taken for the film to become transparent is less. It also shows that when the temperature increases that rate of reaction also increases until it reaches the optimum temperature. This is what I expect will happen to the results in my final experiment. Variables: In this experiment the independent variable will be the temperature, the dependent variable will be the time it takes for the films to become transparent and the controls are: Y Concentration of protease Y Volume of Protease Y Film size The experiment should be carried out the same for each test tube and the pH should stay the same for all test tubes. The concentration of the protease solution will be 0.5% and the volume of each protease solution will be 10cm3. Range: The range of temperatures that I am going to use will be 300C, 400C, 500C, 600C, 700C. If I have a temperature any higher than 700C the enzyme would most probably denature. I havent got a temperature any lower than 300C because it would take too long for the gelatine to break down in the time given. Reliability: In my final experiment I am going to use a syringe to measure out the volume of protease needed. A syringe is accurate enough for this experiment. I will put two developed films into each test tube to improve reliability of my results. I will also use a stopwatch to time when I put the films into the test tube and when to check the films. The electric water baths are really easy to use and they control the variables very precisely unlike heating the test tube with a bunsen burner, as the temperature can go slightly up and down. Safety: Whilst doing the experiment I will have my hair tied back, I will wear a lab coat and I will also wear safety goggles throughout as I am using protease which if gets into your eyes it can be dangerous.

Composition About Film Essay Example for Free

Composition About Film Essay The film that I saw last week is about the dangerous art of extraction valuable secrets from deep within the subconscious during sleep, when the mind is most vulnerable. The main character of the film called Cobb. He is a talented thief, the best of the best in his work. At the beginning of the film we see Cobb’s dream in which he with his wife lived in their town that they create alone in unexpected for me and I was surprising when I saw how in our dream we can see people who not existent in the reality. Then the point is that Cobb’s command received the proposition to change some events in the life of one of the richest company in the world. They had to do that this company fell down and other man who managing other company could be the first and control others. If Cobb, and his command do this he promise Cobb to help him with law, because as we know he was a thief and all police search him and that’s why he can’t returned home to his children. For this work he must to find a new architecture, because without him it will be not possible. Architect a specialist defining illusory world for another dream. The purpose of the architect when extracting designed sleep so sleep could not distinguish it from reality and create a sleeping most complex maze of sleep, from which the victim could not easily escape. The complexity of this work was that this dream consisted of three levels, that is to say they must to reach the deepest within of the subconscious to change what they want. Now their task not to steal an idea, but to implement it. If they succeed, it will be the perfect crime. I realized they done this work in the best way. To my mind this film is very interesting and exciting, but it is necessary to understand . It is fantastical, but as for me it was interesting to see what we can do when we sleep.

Babel Movie Analysis Essay

Babel Movie Analysis Essay The Book of Genesis tells the story of the Tower of Babel. After the Great Flood all of the humankind spoke the same language. People decided to build a city with the tower that can reach heaven. The Tower was built not to worship God, but to make a name for the builders: Then they said, Come, let us build ourselves a city, and a tower with its top in the heavens, and let us make a name for ourselves; otherwise we shall be scattered abroad upon the face of the whole earth. Afterwards, God came down to Earth, and was displeased with the intentions of the builders and the purpose of the Tower of Babel, and he confused their language to break the main principle of their power unity. God also scattered the citizens of Babylon all around the globe. Before going into the analysis of the movie, I would like to make use of the Clifford Geertz argument of cultural interpretations. Social facts and culture are different things, but they are interconnected. If to apply Geertzs terminology we can call a movie director an ethnographer. And whatever the ethnographer researches or studies is highly interpretive to him, to his social facts, culture, norms and values. Culture tends to resist, or is permitted to resist and thus can escape systematic modes of assessment. You either grasp interpretation or you do not, see the point of it or not, accept it or not  [3]  . Therefore, this film in interpreted through the lens of directors perception, so I as a viewer interpret it further through my perception of culture and social facts. The movie Babel was directed by a Mexican director Alejandro Gonzalez. The plot of the movie tells the story of 4 different families American, Moroccan, Mexican and Japanese; and all of these families are interconnected by a series of events and face different social facts imposed by diverse societies with various values and cultural norms. Emile Durkheim defined social fact in two ways. First goes as following A social fact is any way of acting, whether fixed or not, capable of exerting over the individual an external constraint  [4]  . In this definition, Durkheim introduces the idea of social constraint which identifies reasoning behind actions as social facts. Meaning that there are various pressures on an individual on how to act, or of to act in the first place. If he/she violates and breaks this constraint, the consequence of this might be moral (sense of wrongdoing; being judged by people around you) or on the greater scale legal  [5]  . In the movie we can see examples of social constraints to a different extent in all of the 3 story lines. For example, in the beginning of the film the father of the Moroccan family purchases a rifle for his young sons so that they can defend the sheep from jackals. When both of the sons test the rifle, it appeared that younger brother (Yussef) shoots better that the ol der one (Ahmed). This can be interpreted as a social constraint for Ahmed, who felt being judged by the others, even if he was not. What might have given boost to this feeling of judgement is the case of a strong patriarchal rule in Morocco, where more pressure, to some extent, is put on a man. To some level this led to the incident of the bus shooting, where Ahmed was trying to prove that he can shoot better than his younger brother. Another example can be given from this storyline. Yussef was spying on his sister while she was bathing. She knew that her brother was spying, but did nothing to stop it, which on the contrary meant encouragement on some level. This brings the topic of incest taboo to the table. This is constraint which will be not just judged upon, but the legal action can be taken as well. The topic if incest is extremely sensitive and is not tolerated in modern days, although it was acceptable from ancient times in various royal families (e.g. Cleopatras first husband was her brother). Later on, when father found out about what was going on, he physically and verbally punishes children. One of the Durkheim arguments included the way that the children are brought up: Parents impose upon the child ways of seeing, thinking and acting which he himself would not have arrived at spontaneously  [6]  . Further more, Spencer argues that rational education should be reject in favour of giving the child complete freedom. I personally disagree with this view. I do realize that not all of the people are parent material, but this sort view is extreme. Children should be taught certain limitations, rules and values; but the correct balance should be found. As the saying goes Children are their parents reflection. Durkheims second definition was that social fact is general over the whole of a given society whilst having an existence of its own, independent of its individual manifestations. This definition takes more general approach to social facts. In order to better understand this concept Durkheim introduced the term social currents  [7]  which are collective emotions, feelings and thoughts in a group, which have stronger impact than any individuals own emotions. The groups experience originates outside of the individual and moves on to all of the members of the group. Durkheim argues that this has certain coercive effect, meaning that while individuals in the group allow shared experience to strengthen, they also keep an illusion of having created, that which actually forced itself from without  [8]  . Moreover, if an individual separates himself from the group emotion, he will find out that the emotion was not his own and will be met by resistance from the rest of the group, if to attempt to show another felling or emotion. This is exactly what happened to American family in Morocco. American woman got accidentally shot while on the bus tour. Bus driver goes to a nearest village, which only had a veterinarian who sewed the wounds. But the wounded woman still needed full medical care because of severe blood loss, so they were waiting for the ambulance which was driving from the nearest hospital (3 hours away). At the same time other tourists on the bus wanted to leave the woman and her husband (Richard) in the fear of another attack (the first attack was quickly labelled terrorist) and concerns over heat. The tour group and Richard engage in several arguments, eventually resulting in tour group leaving while Richard was on the phone. So if we put this example in previously mentioned Durkheims arguments we can see that after the accident the bus group was divided. The groups experience originated from outside, which in this case was the shooting. But Richard and his wife were quickly separated from the group; because now the group did not share same emotion and the same goals. Consequently, the tour group resisted Richards arguments and left. It is not certain what would have been the outcome of this situation if someone else would have shoot. Every group has a leader, which in the movie was played by a British tourist. What if he got shot? Would have this made a different outcome? Or just actors change and the end result remains the same? I guess the outcome will vary significantly depending on parameters of the situation. The next issue of the movie I would like to address is suicide. We observe the story of a Japanese family Yasujiro and Chieko Wataya. Yasujiro is a widower, whose wife committed suicide and is confronted with communication difficulties with his daughter Chieko, who is deaf mute. Because of Chiekos disability, she is seen as an outcast in the society, with people constantly shutting her down while she is in need of a simple human contact. Later in the movie, she told a police officer that her mother committed suicide by jumping off the balcony, while the truth was that she shot herself. And in the end of the movie we find Chieko standing on the edge as the balcony, which I interpreted as suicide intent. Durkheim wrote extensively on topic of suicide, implementing social current into the issue. He took various approaches such as quantitative and statistical, analysis of weather and season, etc  [9]  . But this data just showed statistics and patters, but not the cause, which in Du rkheims view was social. Durkheim argues that one of the most important aspects for explanation of suicide rates are the degree of integration into and regulation by society. Also depending on the size of the family the larger the family, the lesser the chance of suicide within it. Thus, Japanese families in general (inc. the Watayas) tend to be small in size, which according to Durkheim makes members within them more prone to suicide. Coming back to the role of the society, Durkheim gave a sociological explanation: Each social group really has a collective inclination for the act, quite its own, and the source of all individual inclination, rather than the result. It is made up of the currents of egoism, altruism or anomy running through the society. These tendencies of the whole social body, by affecting individuals, cause them to commit suicide.  [10]  We can see an example of this in the movie, when Chieko and her friend are approached by the boy who invites them for a drink with his friends, but as soon as he realises that both girls are deaf mute, he leaves and laughs with his friends. Such tendencies can cause intent to commit suicide. But what Durkheim does not mention, is how and what people attribute to the actions and behaviour of others, which undermines his theory significantly. Overall, Babel brings out various interesting issues in sociological terms. The movie after all is fictional, and so are the characters; but the social facts and culture are real. Durkheims theories are sound, clear, and are easy to put into real-life situations. But on the other hand, if to completely follow his idea of social facts, our behaviour is more or less predicted by them, and we almost have no option but to accept them. People can do anything they like as long as it does not harm others J.S. Mill On Liberty

Analysis of Royal Caribbean Internationals Operations in Singapore Ess

1. Introduction Deep-sea passenger transportation are amongst the most rapidly growing and evolving sectors in the hospitality industry(Wie, 2004, Douglas et al., 2010). As such, there underscores the need for strategy development to ensure the long-term success of firms in this industry. This report would focus on Royal Caribbean International's operations in Singapore, and analyze it based on its external and internal environments, while concluding with a recommendation of strategies that could be adopted as part of the organization's long-term strategic trajectory. Founded in 1968, the firm is part of the Royal Caribbean Cruises Ltd. which provides one-stop vacation services that includes hotel, dining and entertainment. Additionally, land tours, stopovers and airport transfer services have also been offered in recent years to maximize the customer experience(Royal Caribbean International, n.d.). 2. External Analysis 2.1 PESTLE Analysis Various academics have highlighted the critical role that the PESTLE framework plays in aiding organizations to analyze its external environment(Johnson et al., 2012; Senior and Swailes, 2010; Gomez-Mejia and Balkin, 2011). Political: Owing to the welcoming attitude maintained towards foreign firms, low levels of corruption, stable political climate, and good relations with neighboring countries (MarketLine, 2013), there is little political risk faced in Singapore. Economy: Singapore's economy is relatively stable and growing, albeit being slightly affected by global economic woes. As a whole, there are low levels of unemployment and GDP per capita is high(Worldbank,2013), hence implying more disposable income that nationals have to spend. Nonetheless, spurred on by soaring ... ... Recruiting in the Cruise Industry', Journal of Human Resources in Hospitality and Tourism, 9: 17-32 Gibson, P. and Papathanassis, A. (2010): 'The Cruise Industry – Emerging Issues, Problems and Solutions: Review of the 2nd International Cruise Conference, Plymouth, UK, 18-20 February 2010', International Journal of Tourism Research, 12: 405-7 Thor, J.C. and Barclay, L.A. (2012): 'Art Auctions on the High Seas', Journal of Critical Incidents, 5: 59-61 Wei, Y., Samiee, S., and Lee, R.P. (2014): 'The Influence of Organic Organizational Cultures, Market Responsiveness, and Product Strategy on Firm Performance in An Emerging Market', Journal of the Academy of Marketing Science, 42: 49-70 Wilson, R.H. (2012): 'The Legal Strategy of the Cruise Industry: An Effective Use of Terms and Conditions to Manage Disputes', Cornell Hospitality Quarterly, 53(4): 347-56

Twelve Hours Essay -- Papers

Twelve Hours 6.42 p.m. January 17th The doors signalled the end. They signalled the end of the journey, the end of the obsessive excuse making and theory-formulating process that had lead him here. It was these doors that told him it was over; he had now to face reality. He often considered them as the gates to hell, by the very nature of what goes on behind them. His hatred for the place ran deep; it was a constant occurrence on his timeline that now rested at June 17th, 1989. 1989, he thought to himself. 2 years now this place had been part of his life. Two years of visits, crying and emotional breakdown. Two years of constant fluctuations in his marital life that lead him to question his sanity. The close examination of the degree of sanity that he may possess that had brought him here thus far. Madness? He sometimes thought so. Now, yet again, the outlook was bleak. Was this a chapter in his life that was going to be closed here tonight, on this very godforsaken night? So, here he was, in front of those doors again. I look back fondly. When life was to be lived and where death was something that never touched you or affected you in anyway. Death? Here, yet again I find myself talking about death. Maybe it's the nature of the situation that death is obviously the outcome. Have I a right to speak like this? No? I believe I have. Two years in the spectrum of life is a long time. Wars are fought in less time, friendships forged and relationships ruined. Two years ago, two long years, which now I look back and try and gather some reason for the time I have spent savaged by my own emotions. Often it is the sun dappled path ... ...mself, question why he left it so late to tell the woman he loved just how much he loved her. The scene rests on the moment he laid himself over his wife. We sit still watching the quiet crying of a man in great pain. The memories he held of her would be with him forever. As the scene gently fades into obscurity and the colour is washed out into a grey nostalgic item a sense of calm quells up inside. The loss of human life means so much more than this sentence would suggest. No words can describe what emotions we have without someway distorting the truth and removing the uniqueness and individuality of the love. That is why I will not end this story with a common and mediocre ending, for it deserves so much more. That is why I will leave it up to you and let you decide an ending that is justified and meaningful to you.

Are Books Better than Films? Essays -- new images, overlook of material

According to the article, â€Å" Are Books Better than Films?†, â€Å"Films can bring whole worlds to life before our eyes, make characters into living, breathing fleshing blood, but books let you live everything† (â€Å"Are Books Better than Films?†). Very often book lovers are unsatisfied with the movie adaption of their favorite book. No doubt there have been great book based movies but that does not take the place of reading the actual novel. Reading books is better than watching the movie. This is better because often the movie tends to misinterpret information, which changes the plot, and lack sufficient character development. One of the many famous movie adaptions is from the teen novel series The Twilight Saga. Written by Stephanie Meyer, it has been extremely successful. The series is about a male vampire (Edward Cullen) and human female (Isabella Swan) who fall in love and struggle to keep their relationship. Teens ran out and got the books hot off the press and staked out movie theaters the first weekend it came out. The first weekend the movie came out in theatres it made over $69,637,740. It also ranked the number one movie in 3,419 across America ("Twilight (2008) - Box Office Mojo."). In movie versions, the director seems to overlook material that the reader finds vital to the plot. In the first Twilight book, when Edward and Bella get out of the car he does not touch her because he does not want to risk losing control (Meyer). In the movie, Directed by Catherine Hardwicke, he swings his arm around Bella shoulders and states he was going to hell anyways (Hardwicke). When the director did this he changed the plot because in the book none of the other characters knew Edward and Bella talked. Then all of a sudden Edward and Bella w... ...the chance to increase vocabulary and knowledge. Movies do not equate to books because they lack the following criteria. They do not have full character analysis as the book does nor do they stick to the story line of the book. Directors tend to take their creative liberties too far and begin to alter the plot and create new images of the story. Readers would love to see a great novel come to life but keep it the way the book has written it. "Are Books Better than Films?" Theguardian.com. Guardian News and Media, 06 Nov. 2013. Web. 17 Mar. 2014. "Are Movies Better than Books?" The Premier Online Debate Website. N.p., n.d. Web. 17 Mar. 2014. Meyer, Stephanie. Twilight. London: Atom, 2007. Print. "Twilight (2008) - Box Office Mojo." Twilight (2008) - Box Office Mojo. N.p., n.d. Web. 13 Mar. 2014. Twilight. Dir. Catherine Hardwicke. Paramount Pictures,2008. Film.

Sustainability – Case Study: Zimbabwe

Sustainable development is development that meets the needs of the present generation without compromising the ability of future generations to meet their own needs. Locate and identify with a sketch map the geography of Zimbabwe. Zimbabwe is a landlocked country located in southern eastern Africa. The terrain is mostly high plateau with a higher central plateau (high veld – any of the open grazing areas of southern Africa) and mountains in the east. Zimbabwe covers an area of 39000 sq. kms and has a population of 12.8 million of which 35% are urbanised. Half of these are in the capital city which is Harare (located by the red dot on the map). What are the challenges that face Zimbabwe today? You can read also Costco Case Study There is racial divide as only 2% of the population is white; of the majority black population 71% are Shona and 16% Ndebele. The country is now effectively bankrupt. Social service provision is in decline and unemployment is running at 60%. From being self sufficient in basic food stuffs it is estimated that in 2003/2004 only one third of its main staple food maize will be available. Half the population are said by an O.E.C.D. Report (2003) to be facing starvation without outside aid. There are spatial inequalities such as at the periphery. The World Bank (1999) estimated the 6 million population in the communal lands live on less than an average of 1US$ a head per day and that 58% live under the poverty line compared with 8% in the core. The periphery lacks urbanisation, rail and road networks, power grids, mining estates, manufacturing and various forms of social infrastructure. From 1990 – to date the government moved away from a direct attempt to address the issue of regional inequalities towards economic policies which benefited the core region but which it was hoped would also bring spread effects to the periphery. The core is still dominated by a capitalist based and export orientated economy. There is extreme social dualism, 20% of the population command 60% of the country's GDP and are mainly urban based. Since 2000 an ill thought out Fast Track land reform programme, together with illegal land occupation of white commercial farms, has failed to significantly relieve pressure on communal lands and crippled the commercial agriculture sector which was a main source of food supply and the major source of exports. Outline the urgent priorities that Zimbabwe faces today. Reduction of regional inequalities is a central policy under the government's declared principle of ‘Equity with Growth'. They are a result of a combination of interrelated factors: Natural resource disparities and natural events such as periodic drought, Economic forces, core periphery contrasts, a failure of government policies, external factors operating from 1890. From 1998, and particularly following the controversial 2000 general election, Zimbabwe has been plunged into crisis as a result of a number of factors – periodic droughts, the Aids epidemic, rampant inflation adversely affecting both internal finances and the country's competitiveness in overseas markets and a collapse in all major sectors of the economy. The crippled commercial agriculture sector which was a main source of food supply and the major source of exports, brought about by the land reform programme. This contributes to widespread food shortages and economic downturn from exports.